rat comp elisa kit  (FineTest Biotech Inc)

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Therapeutic effects of combining curcumin and swimming in osteoarthritis using a rat model.

doi: 10.1016/j.biopha.2023.115309

Figure Lengend Snippet: Fig. 4. Relative expression of A) miR-130a and B) HDAC3 in the knee joint homogenate of the rats subjected to 4 weeks of swimming, curcumin or swimming combined with curcumin. One-way ANOVA was used as a statistical test followed by Tukey post-hoc test for significance. ns: non-significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, n = 6/group.

Article Snippet: In addition, cartilage oligomeric matrix protein (COMP) and peroxisome proliferator-activated receptor γ (PPAR-γ) were measured in the knee joint homogenate according to manufacturer’s instructions (Cat. # CSBE13833r, CSB-E08624r, respectively; CusaBio).

Techniques: Expressing

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Therapeutic effects of combining curcumin and swimming in osteoarthritis using a rat model.

doi: 10.1016/j.biopha.2023.115309

Figure Lengend Snippet: Fig. 5. Expression of MMP1 and MMP13 in the knee joint homogenate of the rats subjected to 4 weeks of swimming, curcumin or swimming combined with curcumin shown in the upper pannel. Beta-actin confirms equal loading of the protein. Western blot was performed in triplicates with similar results. One representative blot is shown. One-way ANOVA was used as a statistical test followed by Tukey post-hoc test for significance ns; non-significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, n = 6/group.

Article Snippet: In addition, cartilage oligomeric matrix protein (COMP) and peroxisome proliferator-activated receptor γ (PPAR-γ) were measured in the knee joint homogenate according to manufacturer’s instructions (Cat. # CSBE13833r, CSB-E08624r, respectively; CusaBio).

Techniques: Expressing, Western Blot

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Therapeutic effects of combining curcumin and swimming in osteoarthritis using a rat model.

doi: 10.1016/j.biopha.2023.115309

Figure Lengend Snippet: Fig. 6. Levels of A) PPAR-γ, B) NF-κB, C) IL-1β, and D) TNF-α in the knee joint homogenate of the rats subjected to 4 weeks of swimming, curcumin or swimming combined with curcumin. One-way ANOVA was used as a statistical test followed by Tukey post-hoc test for significance ns; non-significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 n = 6/group.

Article Snippet: In addition, cartilage oligomeric matrix protein (COMP) and peroxisome proliferator-activated receptor γ (PPAR-γ) were measured in the knee joint homogenate according to manufacturer’s instructions (Cat. # CSBE13833r, CSB-E08624r, respectively; CusaBio).

Techniques:

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: A glucuronated flavone TMMG spatially targets chondrocytes to alleviate cartilage degeneration through negative regulation of IL-1β.

doi: 10.1016/j.biopha.2023.114809

Figure Lengend Snippet: Fig. 8. Effect of TMMG administration on Elevated Serum Markers in ACLT Animals. In the serum, (A) pro-inflammatory cytokine IL-1β, (B) Carboxy-terminal telopeptides CTX-II, and (C) COMP (Cartilage oligomeric matrix protein) were elevated in the ACLT-induced osteoarthritis model. These serum bio-markers are diagnostic markers of osteoarthritis. TMMG regulated the levels of these markers in the ACLT group. All values are expressed as mean ± S.E.M. (n = 4–6/group); *P < 0.05, * *P < 0.01, and * **P < 0.001 vs. control group, and #P < 0.05, ##P < 0.01, and ###P < 0.001 vs. ACLT group.

Article Snippet: The TUNEL assay kit (C10618) was purchased from Molecular Probes Life Technologies, Rat Type II collagen Cterminal cross-linked peptide (CTX-II, E-EL-R2554) and cartilage oligomeric matrix protein (COMP, E-EL-R0159), ELISA kits were purchased from Elabscience (Elabsciences Biotechnology Co., Ltd., Hubei province, China).

Techniques: Diagnostic Assay, Control